Madonna got bold during her performance of "Human Nature" again.

Madonna won't stop at nipple when it comes to exposing her body. Less than a week after giving Istanbul crowd a peek of her breast, the singer flashed her butt to her concert audience in Italy on Tuesday, June 12.

Madonna got bold during her performance of "Human Nature" again. The 53-year-old turned her back to the audience, unzipped her pants and exposed her thong-clad backside to her fans at Rome's Stadio Olimpico.

With her cover of Lady GaGa's "Born This Way", swastika image, nipple and butt flash, Madonna has created enough controversy during her MDNA tour. She will next visit Florence and Milan later this week.

However, not every fellow celebrity condones her act. TV presented Piers Morgan slammed Madonna, calling her nipple act "most embarrassing, cringe-worthy, desperate moment in the history of music?"

Ironically, Madonna herself said back in January during press conference of her film "W.E.", " 'You don't have to show nipples to be interesting. It doesn't necessarily mean you're cutting edge if you do, right?"